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Optimization of process variables for phyllanthin extraction from Phyllanthus amarus leaves by supercritical fluid using a Box-Behnken experimental design followed by HPLC identification

机译:应用Box-Behnken实验设计和HPLC鉴定优化超临界流体提取Ph叶中的叶绿素的工艺变量

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摘要

The response surface methodology using the Box-Behnken design was established to describe supercritical carbon dioxide assisted extraction of phyllanthin from Phyllanthus amarus Schum and Thonn leaves prior to HPLC analysis. The effects of extraction pressure, temperature, modifier concentration and extraction time on the yield of phyllanthin were investigated. By solving the regression equation, the optimum conditions were as follows: extraction pressure 23.2 MPa, temperature 40 °C, methanol as modifier at a concentration 10 % and time 90 min. Under these conditions, the phyllanthin yield was 12.83 ± 0.28 mg g-1, which was in good agreement with the predicted values. Modifier concentration and extraction time showed a significant effect on the phyllanthin yield.
机译:建立了使用Box-Behnken设计的响应面方法,以描述在HPLC分析之前超临界二氧化碳辅助从余甘子叶和Thonn叶中提取叶绿素的方法。研究了提取压力,温度,改性剂浓度和提取时间对叶绿素产量的影响。通过求解回归方程,最佳条件如下:萃取压力为23.2 MPa,温度为40°C,甲醇为改性剂,浓度为10%,时间为90分钟。在这些条件下,phyllanthin的产量为12.83±0.28 mg g-1,与预测值非常吻合。改性剂的浓度和提取时间对叶绿素的产量有显着影响。

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